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Chemical Contaminants in Plant Based Protein Survey

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  • Food Standards Scotland

Chemical Contaminants in Plant Based Protein Survey

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Content guide

Annex C: Tables of results

Table C1. Validation data for plant protein products – 11+ IAC Multimycotoxin IAC method

  AFB1AFB2AFG1AFG2OTASTG
Spike level (n=6)LOQ0.250.250.250.250.250.25

1xLOQ

 

 

Average recovery (%)81.590.085.490.968.874.6
s.d.3.93.15.85.619.42.6
cv (%)4.83.56.76.228.23.5

2xLOQ

 

 

Average recovery (%)85.890.888.695.473.375.8
s.d.3.31.73.12.310.62.4
cv (%)3.81.83.52.414.43.2

10xLOQ

 

 

Average recovery (%)86.590.488.793.277.5*75.6
s.d.3.62.72.73.69.1*3.0
cv (%)4.13.03.13.911.8*4.0
 Overall average recovery84.690.487.693.273.275.3

*one outlying result excluded from this set, sample likely not spiked prior to extraction n=5

Table C1 contd.  Validation data for plant protein products – 11+ IAC Multimycotoxin IAC method
  DONHT2T2ZONFB1FB2FB3
Spike level (n=6)LOQ5552.51055

1xLOQ

 

 

Average recovery (%)99.6112.399.489.078.772.276.2
s.d.4.917.32.13.34.43.43.4
cv (%)4.915.42.13.75.64.74.4

2xLOQ

 

 

Average recovery (%)108.399.8100.387.882.679.879.9
s.d.15.79.11.94.52.72.64.2
cv (%)14.59.11.95.13.33.35.3

10xLOQ

 

 

Average recovery (%)104.996.497.885.086.580.584.5
s.d.5.73.43.53.03.12.31.7
cv (%)5.43.53.53.53.52.92.0
 Overall average recovery104.3102.999.287.382.677.580.2

Table C2. Mycotoxin results for Phase 1 plant protein powders, using 11+ IAC method. Results in µg/kg, corrected for recovery.

Fera Sample No.Product type AFB1AFB2AFG1AFG2OTASTG
S24-069134Pea Powder<0.08<0.08<0.08<0.089.33<0.08
S24-069135Soy Powder<0.08<0.08<0.08<0.083.22<0.08
S24-069136Pea Powder0.15<0.08<0.08<0.08<0.08<0.08
S24-069137Soy Powder2.240.122.460.124.15<0.08
S24-069138Soy Powder<0.08<0.08<0.08<0.08<0.08<0.08
S24-069318Pea Powder<0.08<0.08<0.08<0.08<0.08<0.08
Fera Sample No.Product type DONHT2T2ZONFB1FB2FB3
S24-069134Pea Powder<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069135Soy Powder<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069136Pea Powder<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069137Soy Powder<1.56<1.56<1.561.13<3.125<1.56<1.56
S24-069138Soy Powder<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069318Pea Powder<1.56<1.56<1.56<0.78<3.125<1.56<1.56

 

Table C3. Mycotoxin results for Phase 1 Soy based products, using 11+ IAC method. Results in µg/kg, corrected for recovery.

Fera Sample No.Product type AFB1AFB2AFG1AFG2OTASTG
S24-069319Soy based product <0.08<0.08<0.08<0.08<0.08<0.08
S24-069320Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069321Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069322Soy based product0.22<0.08<0.08<0.080.39<0.08
S24-069323Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069882Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069883Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069884Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069890Soy based product<0.08<0.08<0.08<0.081.85<0.08
Fera Sample No.Product type DONHT2T2ZONFB1FB2FB3
S24-069319Soy based product <1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069320Soy based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069321Soy based product<1.56<1.56<1.56<0.7824.703.743.31
S24-069322Soy based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069323Soy based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069882Soy based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069883Soy based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069884Soy based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069890Soy based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56

 

Table C4. Mycotoxin results for Phase 1 Pea based products, using 11+ IAC method. Results in µg/kg, corrected for recovery.

Fera Sample No.Product type AFB1AFB2AFG1AFG2OTASTG
S24-069324Pea based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069885Pea based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069886Pea based product<0.08<0.08<0.08<0.080.53<0.08
S24-069887Pea based product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069888Pea based product<0.08<0.08<0.08<0.080.26<0.08
S24-069889Pea based product<0.08<0.08<0.08<0.080.54<0.08
Fera Sample No.Product type DONHT2T2ZONFB1FB2FB3
S24-069324Pea based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069885Pea based product<1.56<1.56<1.561.75<3.125<1.56<1.56
S24-069886Pea based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069887Pea based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069888Pea based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069889Pea based product<1.56<1.56<1.56<0.78<3.125<1.56<1.56

Table C5. Mycotoxin results for Phase 1 mixed plant protein products, using 11+ IAC method. Results in µg/kg, corrected for recovery.

Fera Sample No.Product type AFB1AFB2AFG1AFG2OTASTG
S24-069325Soy & wheat product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069326Pea, wheat and soy product<0.08<0.08<0.08<0.080.320.25
S24-069327Soy & wheat product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069328Soy & wheat product<0.08<0.08<0.08<0.08<0.08<0.08
S24-069891Soy & wheat product<0.08<0.08<0.08<0.080.11<0.08
Fera Sample No.Product type DONHT2T2ZONFB1FB2FB3
S24-069325Soy & wheat product7.35<1.56<1.56<0.78<3.125<1.56<1.56
S24-069326Pea, wheat and soy product3.76<1.56<1.56<0.78<3.125<1.56<1.56
S24-069327Soy & wheat product<1.56<1.56<1.56<0.78<3.125<1.56<1.56
S24-069328Soy & wheat product3.07<1.56<1.561.1<3.125<1.56<1.56
S24-069891Soy & wheat product2.85<1.56<1.56<0.78<3.125<1.56<1.56

Table C6. Quality control data for Phase 1 11+ IAC mycotoxin method analysis. Values reported are % Recovery.

 AFB1AFB2AFG1AFG2OTASTG
Target LOQ (µg/kg)0.250.250.250.250.250.25
 Recovery (%)
Sp 1x LOQ Powder72.382.878.086.250.263.5
Sp 2x LOQ Powder86.994.587.990.883.075.3
Sp 10x LOQ Powder87.593.290.593.986.973.9
Average Powder Sp82.290.285.590.373.470.9
       
Sp 1x LOQ Soy Tofu84.179.783.181.184.673.9
Sp 2x LOQ Soy Tofu88.192.090.695.078.775.5
Sp 10x LOQ Soy Tofu88.494.193.297.381.675.9
Average Soy Sp86.988.689.091.281.675.1
       
Sp 1x LOQ Pea product81.193.279.982.074.672.6
Sp 2x LOQ Pea product92.696.496.394.870.577.7
Sp 10x LOQ Pea product89.697.093.397.382.277.4
Average Pea product Sp87.795.689.891.475.875.9
       
Sp 1x LOQ Mixed92.592.090.894.387.180.4
Sp 2x LOQ Mixed90.093.288.397.186.080.1
Sp 10x LOQ Mixed90.092.090.490.679.180.5
Average Mixed Sp90.892.489.894.084.180.4

Table C6. contd. Quality control data for 11+ IAC mycotoxin method analysis. Values reported are % recovery.

 DONHT2T2ZONFB1FB2FB3
Target LOQ (µg/kg)5552.51055
 Recovery (%)        
Sp 1x LOQ Powder89.990.188.578.679.475.075.1
Sp 2x LOQ Powder107.198.098.188.789.385.889.8
Sp 10x LOQ Powder97.6100.199.882.692.286.089.1
Average Powder Recovery98.296.195.583.387.082.384.7
        
Sp 1x LOQ Soy Tofu85.591.591.277.758.954.857.1
Sp 2x LOQ Soy Tofu99.585.894.891.063.058.463.1
Sp 10x LOQ Soy Tofu94.894.595.587.381.774.073.9
Average Soy Recovery93.390.693.885.367.962.464.7
        
Sp 1x LOQ Pea product98.181.492.192.452.856.074.2
Sp 2x LOQ Pea product101.488.2104.997.560.460.771.2
Sp 10x LOQ Pea product102.995.6102.593.078.072.975.3
Average Pea product Recovery100.888.499.894.363.763.273.5
        
Sp 1x LOQ Mixed104.685.7101.896.486.575.272.7
Sp 2x LOQ Mixed115.297.398.688.384.579.276.4
Sp 10x LOQ Mixed94.190.194.987.480.374.781.1
Average Mixed Recovery104.691.098.490.783.876.476.7

Table C7. Mycotoxin results for Phase 2 plant protein powders, using 11+ IAC method. Results in µg/kg, corrected for recovery

Analytical Batch PC25-04914

Fera Sample NumberProduct typeAFB1AFB2AFG1AFG2OTASTG
S25-037296Pea protein powder <0.08<0.08<0.08<0.08<0.08<0.08
S25-037297Soy protein powder<0.08<0.08<0.08<0.080.44<0.08
S25-037298Pea protein powder <0.08<0.08<0.08<0.082.11<0.08
S25-037299Pea & fava protein powder0.34<0.08<0.08<0.080.920.20
S25-037300Pea & soy protein powder0.11<0.08<0.08<0.080.41<0.08
S25-037301Vegan blend protein powder<0.08<0.08<0.08<0.080.46<0.08
S25-037454Whey & soy protein powder0.63<0.080.29<0.081.70<0.08
S25-037455Pea protein shake powder <0.08<0.08<0.08<0.082.27<0.08
S25-037459Pea & rice protein powder0.44<0.08<0.08<0.080.371.55
Fera Sample NumberProduct typeDONHT2T2ZONFB1FB2FB3
S25-037296Pea protein powder <1.56<1.56<1.56<0.78<3.13<1.56<1.56
S25-037297Soy protein powder<1.56<1.56<1.56<0.78<3.13<1.56<1.56
S25-037298Pea protein powder <1.56<1.56<1.56<0.78<3.13<1.56<1.56
S25-037299Pea & fava protein powder<1.56<1.56<1.56<0.78<3.13<1.56<1.56
S25-037300Pea & soy protein powder<1.56<1.56<1.562.80<3.13<1.56<1.56
S25-037301Vegan blend protein powder<1.56<1.56<1.56<0.78<3.13<1.56<1.56
S25-037454Whey & soy protein powder<1.56<1.56<1.562.5614.915.89<1.56
S25-037455Pea protein shake powder 3.432.45<1.56<0.78<3.13<1.56<1.56
S25-037459Pea & rice protein powder<1.56<1.56<1.561.06<3.13<1.56<1.56

 

Table C8. Mycotoxin results for Phase 2 meat replacement products, using 11+ IAC method. Results in µg/kg, corrected for recovery

Analytical Batch PC25-04661

Fera Sample NumberProduct typeAFB1AFB2AFG1AFG2OTASTG
S25-037315Soy product<0.08<0.08<0.08<0.08<0.08<0.08
S25-037316Pea based product<0.08<0.08<0.08<0.08<0.08<0.08
S25-037317Soy and pea based product<0.08<0.08<0.08<0.080.32<0.08
S25-037318Pea based product <0.08<0.08<0.08<0.080.22<0.08
S25-037319Wheat and pea based product <0.08<0.08<0.08<0.080.130.33
S25-037320Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S25-037339Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S25-037340Soy based product<0.08<0.08<0.08<0.08<0.08<0.08
S25-037341Pea based product0.12<0.08<0.08<0.080.23<0.08
S25-037342Pea based product<0.08<0.08<0.08<0.08<0.08<0.08
Fera Sample NumberProduct typeDONHT2T2ZONFB1FB2FB3
S25-037315Soy product<1.56<3.13<1.561.28i<3.13<1.56<1.56
S25-037316Pea based product<1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037317Soy and pea based product<1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037318Pea based product <1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037319Wheat and pea based product11.22<3.13<1.56<0.78<3.13<1.56<1.56
S25-037320Soy based product<1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037339Soy based product<1.56<3.13<1.561.88<3.13<1.56<1.56
S25-037340Soy based product<1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037341Pea based product<1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037342Pea based product<1.56<3.13<1.56<0.78<3.13<1.56<1.56

i indicates result did not confirm by ion ratio, uncorrected concentration was at the LOQ 

Table C9. Mycotoxin results for Phase 2 ready to drink protein shake products and protein bars, using 11+ IAC method. Results in µg/kg, corrected for recovery

Analytical Batch PC25-04661

Fera Sample NumberProduct typeAFB1AFB2AFG1AFG2OTASTG
S25-037456RTD protein shake<0.08<0.08<0.08<0.08<0.08<0.08
S25-037457RTD protein shake0.23<0.08<0.08<0.08<0.08<0.08
S25-037458RTD protein shake<0.08<0.08<0.08<0.08<0.08<0.08
S25-037302Protein & cereal bar <0.08<0.08<0.08<0.08<0.08<0.08
S25-037303Protein & cereal bar 0.15<0.08<0.08<0.080.55<0.08
S25-037304Protein & cereal bar <0.08<0.08<0.08<0.080.390.22
S25-037453Protein & cereal bar <0.08<0.08<0.08<0.080.390.54
Fera Sample NumberProduct typeDONHT2T2ZONFB1FB2FB3
S25-037456RTD protein shake<1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037457RTD protein shake14.55<3.13<1.562.08<3.13<1.56<1.56
S25-037458RTD protein shake2.32<3.13<1.56<0.78<3.13<1.56<1.56
S25-037302Protein & cereal bar <1.564.16<1.56<0.78<3.13<1.56<1.56
S25-037303Protein & cereal bar <1.56<3.13<1.56<0.78<3.13<1.56<1.56
S25-037304Protein & cereal bar 2.44<3.13<1.561.67<3.13<1.56<1.56
S25-037453Protein & cereal bar 8.32<3.13<1.564.85<3.13<1.56<1.56

 

Table C10. Quality control data for Phase 2 11+ mycotoxin method analysis.

Batch PC25-04914 – protein powders

Recovery (%)AFB1AFB2AFG1AFG2DONFB1FB2FB3HT2OTASTGT2ZON
Spike level (µg/kg)2.52.52.52.5501005050502.52.55025
Sp176.685.781.483.3106.580.075.477.589.284.164.093.573.7
Sp279.385.884.085.8104.284.680.382.487.882.163.495.974.9
Sp3 88.191.491.292.185.586.987.687.887.486.074.797.378.5
Sp4 71.783.280.588.6101.484.381.089.294.180.937.7104.867.6
Average78.986.584.387.499.483.981.184.289.683.360.097.973.7

Batch PC25-04661 – vegan meat replacement products, protein shakes and cereal & protein bars

Recovery (%)AFB1AFB2AFG1AFG2DONFB1FB2FB3HT2OTASTGT2ZON
Spike level (µg/kg)2.52.52.52.5501005050502.52.55025
Vegan meat Sp188.688.889.687.990.986.781.283.291.882.959.297.077.2
Vegan meat Sp287.185.586.987.995.584.879.782.091.489.662.397.279.3
Average 87.887.188.287.993.285.780.482.691.686.360.897.178.3
              
Vegan milk Sp180.079.775.679.687.583.588.384.486.383.853.694.577.6
Vegan milk Sp278.385.887.884.174.385.082.886.798.692.769.195.975.1
Vegan milk Sp393.793.292.093.989.2104.1101.9102.898.284.474.5104.389.7
Average84.086.285.185.983.690.891.091.394.487.065.798.280.8
              
Cereal Sp187.094.794.590.093.5100.9102.0100.0102.673.465.9110.588.4
Cereal Sp287.688.288.590.592.197.492.894.0100.590.862.8104.976.7
Cereal Sp385.086.387.888.295.192.094.693.595.894.066.999.382.7
Cereal Sp482.979.182.781.896.974.478.579.492.174.358.196.079.8
Average 85.687.188.487.694.491.291.991.797.783.163.4102.781.9

Table C11. Validation data for ergot alkaloids

Spike level* ErgocornineErgocorninineErgocristineErgocristininea-Ergocryptineb-Ergocryptine#a+b 
Ergocryptinine

1xLOQ
(n=6)

 

 

Average recovery (%)76.184.879.5100.976.6-100.3
s.d.3.02.83.41.94.1-3.3
cv (%)4.03.34.21.95.4-3.2

2xLOQ
(n=5)

 

 

Average recovery (%)75.178.173.889.574.9-91.9
s.d.1.90.61.71.83.1-2.0
cv (%)2.50.82.22.04.1-2.2

10xLOQ
(n=6)

 

 

Average recovery (%)76.378.176.686.577.0-90.3
s.d.1.72.82.22.32.0-1.6
cv (%)2.33.62.82.72.6-1.8
 Overall Average recovery75.980.576.892.576.2-94.3

*LOQ for individual ergot alkaloids was 0.5 µg/kg each.

# no analytical standard available for b-Ergocryptine so cannot be added as spike. Results in samples quantified from a-Ergocryptine. 

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